TruSeq Stranded Total RNA Library Prep Kit

"Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin High Throughput (96 samples, 96 indexes)/RS-12

產(chǎn)品編號： RS-122-2503

美  元  價： $10280.00

品       牌： Illumina

產(chǎn)       地： 美國

公       司： Illumina, Inc.

Product Highlights:

TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver a clear and comprehensive view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.

High-quality whole-transcriptome analysis with precise strand information

These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:

Uniform coverage and precise measurement of strand orientation

High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression

Discovery across both coding and multiple forms of non-coding RNA

Efficient ribosomal RNA and globin mRNA removal in a single step

Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

TrADItional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Find an up-to-date list of high-throughput automation vendors with robotic systems compatIBLe with this library preparation kit

Specifications:

Input Quantity 0.1 – 1 ug high-quality purified total RNA from blood

Content Specifications Captures coding RNA plus multiple forms of non-coding RNA

Mechanism of Action Bead-based rRNA depletion, CDNA synthesis, and PCR

Multiplexing Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples

System Compatibility NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000

Automation CapABIlity Liquid Handling Robots

Variant Class Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions

Species Category Rat,Mouse

Method Whole-Transcriptome Sequencing

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